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KILR® Retroparticles for Suspension Cells (Hygromycin B)

97-0006

4 Vials x 1.0 mL

KILR® Retroparticles for Suspension Cells (Hygromycin B) contains MMLV particles, that can transduce a wide variety of suspension cells with a housekeeping gene tagged with ProLabel® (ePL), a β-gal reporter fragment. This results in a high level of expression of the fusion protein inside the target cells. Target cell death in a cytotoxicity assay results in the release of the ePL-tagged protein into the medium. Addition of KILR detection reagent, containing the complementing β-gal reporter fragment, Enzyme Acceptor (EA), results in complementation of the two enzyme fragments (EA and ePL). The resulting functional enzyme hydrolyzes a substrate to generate a chemiluminescent signal.

KILR® Retroparticles (HYGRO) Suspension Part #30-566