Gene was obtained from mouse DNA expressed in E. coli modified as described in Day, et al. (1992) "Engineered disulfide bond greatly increases specific activity of recombinant murine interferon beta" (J. Interferon Res. 12: 139-43).
純度
> 95% by SDS-PAGE stained by Coomassie Blue Endotoxin level < 1 EU/μg
生物活性
Measured using a cytopathic effect inhibition assay on Mouse (L929) cells with EMCV. EC50 for IFNβ is ~ 2.5U/ml
緩沖液
20 mM HEPES, pH 6.0; 0.5M NaCl; 6% glycerol; 0.1% bovine serum albumin (BSA)
內(nèi)毒素
< 1 EU/μg
純度
> 95%
純化方法
A combination of ion exchange, hydrophobic interaction and size exclusion chromatography
貯存
For retention of full activity store at -70 oC or below and avoid repeated freeze-thaw cycles
產(chǎn)品特點(diǎn):
E.coli expressed Mouse IFN Beta protein with a single non-glycosylated polypeptide chain
Increased stability with engineered disulfide bond