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Concentration
5u/μl
5'… CCANNNN↓NTGG…3' 
3'…GGTN↑NNNNACC…5

Reaction Conditions 
1X Buffer V2
10mM Tris-HCl (pH7.5 at 30°C), 10mM MgCl₂, 50mM NaCl, and 100μg /ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 5-fold overdigestion with AccB7 I, 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 2.5u of AccB7I for 16 hours at 37°C.
Supplied with 10X Buffer V2, 10X Buffer UB and Viva Buffer A. (Diluent) 
High enzyme concentration may result in Star Activity.
*Blocked by overlapping dcmmethylation (C^mCWGG): CCANNNCCTGG or CCAGGNNNTGG

Ordering Information

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Manual
AccB7 I {PflMI}